Journal: Scientific Reports

Article Title: Conventional and Specific-Pathogen Free Rats Respond Differently to Anesthesia and Surgical Trauma

doi: 10.1038/s41598-019-45871-z

Figure Lengend Snippet: Representative lead II electrocardiograms (ECG) from SPF ( a ) and Conventional ( b ) male Sprague Dawley rats taken under isoflurane anesthesia following surgical instrumentation (n = 20 each group). SPF rats were arrhythmogenic with incidences of ventricular tachycardia, Salvos, bigeminy, and premature ventricular contractions. See Table for data.

Article Snippet: Electrocardiograms were analysed using the LabChart Pro ECG Analysis module (AD Instruments) with rat beat detection settings and Bazett correction for QT.

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Journal: Scientific Reports

Article Title: Conventional and Specific-Pathogen Free Rats Respond Differently to Anesthesia and Surgical Trauma

doi: 10.1038/s41598-019-45871-z

Figure Lengend Snippet: Episodes of arrhythmias and ECG parameters of male Sprague-Dawley Conventional and SPF rats after 7 days acclimation in the laboratory prior to experimentation.

Article Snippet: Electrocardiograms were analysed using the LabChart Pro ECG Analysis module (AD Instruments) with rat beat detection settings and Bazett correction for QT.

Techniques:

Journal: eNeuro

Article Title: Enlarged Interior Built Environment Scale Modulates High-Frequency EEG Oscillations

doi: 10.1523/ENEURO.0104-22.2022

Figure Lengend Snippet: Experimental design and setup. A , Isometric view of conditions. Participants were presented with eyes open resting state, followed by four randomized scale conditions. Each scene lasted 2 min, between which the resting state was displayed while the participant completed a self-report assessment of emotion. B , Floor plan indicating the position of items in the experiment. IEQ variables were measured continuously, and all recording equipment was positioned outside of the participants field of view. C , Diagram of equipment fitted to participant including stereoscopic tracking glasses, EEG system, respiratory belt, SCR finger cradles, and ECG electrodes. Diagrams are representative, not drawn to exact scale.

Article Snippet: A 10 min, three-lead ECG recording (PowerLab, LabChart Pro 8.1.16) was performed.

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Journal: Science translational medicine

Article Title: Exercise triggers CAPN1-mediated AIF truncation, inducing myocyte cell death in arrhythmogenic cardiomyopathy

doi: 10.1126/scitranslmed.abf0891

Figure Lengend Snippet: (A) Percent survival during swim. *P < 0.05 compared to WT, Mantel-Cox survival analysis. (B) Representative long-axis (top) and two-dimensional (2D), B-mode (bottom two panels) echocardiography from exercised WT and Dsg2mut/mut mice. Data are representative of n ≥ 15 mice per genotype. Yellow-dashed box indicates enlarged image of 2D, B-mode middle. (C) Comparison of the percent RV ejection fraction (%RVEF) with the percent left ventricle ejection fraction (%LVEF) using Pearson’s correlation analysis. P values represent Pearson’s correlation between %RVEF and %LVEF within each genotype (n ≥ 12 mice per genotype per parameter). (D and E) Representative HMGB1 immunostained myocardium from exercised WT and Dsg2mut/mut mice. White arrows, cardiomyocytes (CMs) positive (+) for HMGB1 nuclear localization; yellow arrows, non-CMs with HMGB1+ nuclei; white arrowheads, cytoplasmic HMGB1. In (E), dotted-line white boxes highlight enlarged areas in the right panels with the upper boxed area shown in the first panel to the right and the lower boxed area shown in the second panel to the right. Yellow scale bar in the first panel (E), 100 μm; white scale bars, 20 μm. (F) Quantification of CMs and non-CMs positive for nuclear HMGB1 in myocardium from exercised Dsg2mut/mut and WT mice. Data are presented as means ± SEM (n = 7 mice per genotype per parameter; *P < 0.05 Dsg2mut/mut CMs compared to WT CMs; †P < 0.05 Dsg2mut/mut non-CMs compared to WT non-CMs using paired t test).

Article Snippet: All ECG parameters were analyzed following previously described guidelines ( 100 ), using the LabChart Pro ECG Analysis Add-on Software (LabChart Pro 8, MLS360/8, ADInstruments).

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